pMSCV-KD-mAbl
(Plasmid #69028)

• Purpose: Retorviral vector encoding Kinase Defective mouse c-Abl
• Depositing Lab: Jean Wang
• Publication: Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi: 10.1371/journal.pone.0077495. eCollection 2013.

Backbone

• Vector backbone: pMSCV-hygro
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 10393
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse c-Abl Type VI - kinase defective
• Species: M. musculus (mouse)
• Insert Size (bp): 3429
• Mutation: The KD (kinase defective) mutation is at Lysine 290 (K290H). This creates an ApaLI site in the KD mutant that is not in the WT construct.
• Tag / Fusion Protein:
  • FLAG tag inserted at Internal SalI site (2934 bp)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry