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Addgene

pRK5-HA-GST-4EBP1-Low GC
(Plasmid #69018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EIF4EBP1
  • Species
    H. sapiens (human)
  • Mutation
    lowGC content, wt is ~70% GC rich. Still, it codes for WT human 4EBP.
  • Entrez Gene
    EIF4EBP1 (a.k.a. 4E-BP1, 4EBP1, BP-1, PHAS-I)
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer pRK5 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-GST-4EBP1-Low GC was a gift from David Sabatini (Addgene plasmid # 69018 ; http://n2t.net/addgene:69018 ; RRID:Addgene_69018)

  • For your References section:

    A diverse array of cancer-associated MTOR mutations are hyperactivating and can predict rapamycin sensitivity. Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan A, Beck AH, Sabatini DM. Cancer Discov. 2014 May;4(5):554-63. doi: 10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14. 10.1158/2159-8290.CD-13-0929 PubMed 24631838
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