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Addgene

pcDNA3-FLAG-MTOR-F1888L
(Plasmid #69010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 13254
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MTOR-F1888L
  • Alt name
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7650
  • Mutation
    change Phenylalanine 1888 to Leucine
  • GenBank ID
    NM_004958
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    AddGene pcDNA3-Flag mTOR wt (Plasmid #26603)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-MTOR-F1888L was a gift from David Sabatini (Addgene plasmid # 69010 ; http://n2t.net/addgene:69010 ; RRID:Addgene_69010)

  • For your References section:

    A diverse array of cancer-associated MTOR mutations are hyperactivating and can predict rapamycin sensitivity. Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan A, Beck AH, Sabatini DM. Cancer Discov. 2014 May;4(5):554-63. doi: 10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14. 10.1158/2159-8290.CD-13-0929 PubMed 24631838
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