pcDNA3-FLAG-MTOR-E1799K
(Plasmid #69009)

• Purpose: activating MTOR mutation
• Depositing Lab: David Sabatini
• Publication: Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi: 10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 13254
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MTOR-E1799K
• Alt name: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1
• Species: H. sapiens (human)
• Insert Size (bp): 7650
• Mutation: change Glutamate 1799 to Lysine
• GenBank ID: NM_004958
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: AddGene pcDNA3-Flag mTOR wt (Plasmid #26603)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.