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Purposemammalian expression of human p53 S15A mutant.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep53
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SpeciesH. sapiens (human)
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MutationS15A
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 p53 S15A was a gift from David Meek (Addgene plasmid # 69004 ; http://n2t.net/addgene:69004 ; RRID:Addgene_69004) -
For your References section:
Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters. Loughery J, Cox M, Smith LM, Meek DW. Nucleic Acids Res. 2014 Jul;42(12):7666-80. doi: 10.1093/nar/gku501. Epub 2014 Jun 13. 10.1093/nar/gku501 PubMed 24928858
