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mhc2dab:GFP-LT
(Plasmid #68996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mTol2-GFP-LT
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mhc2dab
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3800
  • GenBank ID
    AL928944.8
  • Entrez Gene
    mhc2dab (a.k.a. DAB, DAB1, dab1*01, dab101, dab2*01, dab201, dab3*01, dab301, dab4*01, dab4*02, dab401, dab402, mhc2d8.37b3, mhc2deb, si:dkeyp-2h4.1, zgc:110349, zgc:152682)
  • Promoter mhc2dab
  • Tag / Fusion Protein
    • GFP-LT (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer GAGCGGCCGCCTTAGTGTATGTACGAGTGTATAGATGTTTCCC
  • 3′ sequencing primer GAGGATCCGAGTCTTTGAATGTGTCAAATGAAGAACTTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 3.8-kb fragment upstream of the mhc2dab transcriptional start site was amplified from the bacmid AL928944

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mhc2dab:GFP-LT was a gift from David Traver (Addgene plasmid # 68996 ; http://n2t.net/addgene:68996 ; RRID:Addgene_68996)

  • For your References section:

    Characterization of the mononuclear phagocyte system in zebrafish. Wittamer V, Bertrand JY, Gutschow PW, Traver D. Blood. 2011 Jun 30;117(26):7126-35. doi: 10.1182/blood-2010-11-321448. Epub 2011 Mar 15. 10.1182/blood-2010-11-321448 PubMed 21406720
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