pIMAY
(Plasmid #68939)

• Purpose: (Empty Backbone) E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange
• Depositing Lab: Tim Foster
• Publication: Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. Print 2012.

Backbone

• Vector backbone: N/A
• Backbone size (bp): 5743
• Vector type: Staphylococcal allelic exchange vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

It is prudent to check that the pIMAY is still temperature sensitive in Staph Aureus before starting any cloning. The author found out that it could lose this function while cultured in Ecoli. That's why Ecoli bearing pIMAY MUST be cultured at 37C in Chloramphenicol (10µg/ml).