pAT891
(Plasmid
#68932)
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PurposeInt8 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNBU2
- Total vector size (bp) 6680
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Vector typeBacterial Expression
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Selectable markersErythromycin in Bacteroides
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAT891 was a gift from Timothy Lu & Christopher Voigt (Addgene plasmid # 68932 ; http://n2t.net/addgene:68932 ; RRID:Addgene_68932) -
For your References section:
Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Mimee M, Tucker AC, Voigt CA, Lu TK. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. 10.1016/j.cels.2015.06.001 PubMed 26918244