pMM659
(Plasmid
#68888)
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PurposeChondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, AmpR
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68888 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNBU2
- Total vector size (bp) 5658
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Vector typeBacterial Expression
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Selectable markersErythromycin in Bacteroides
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNanoLuc
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Insert Size (bp)516
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM659 was a gift from Timothy Lu & Christopher Voigt (Addgene plasmid # 68888 ; http://n2t.net/addgene:68888 ; RRID:Addgene_68888) -
For your References section:
Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Mimee M, Tucker AC, Voigt CA, Lu TK. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. 10.1016/j.cels.2015.06.001 PubMed 26918244
