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pLVX-Flag-CDK19-WT-IRES-ZsGreen
(Plasmid #68858)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68858 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-EF1α-IRES-ZsGreen
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8892
  • Total vector size (bp) 10449
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclin-Dependent Kinase 19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1557
  • GenBank ID
    NM_015076
  • Entrez Gene
    CDK19 (a.k.a. CDC2L6, CDK11, DEE87, EIEE87, bA346C16.3)
  • Promoter EF1α
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    F-CDK8L Plasmid #24762

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Flag-CDK19-WT-IRES-ZsGreen was a gift from Matthew Shair (Addgene plasmid # 68858 ; http://n2t.net/addgene:68858 ; RRID:Addgene_68858)

  • For your References section:

    Mediator kinase inhibition further activates super-enhancer-associated genes in AML. Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, Taatjes DJ, Shair MD. Nature. 2015 Sep 28. doi: 10.1038/nature14904. 10.1038/nature14904 PubMed 26416749
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