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pLVX-Flag-CDK8-W105M-IRES-mCherry
(Plasmid #68857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-EF1α-IRES-mCherry
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8865
  • Total vector size (bp) 10311
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclin-Dependent Kinase 8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1446
  • Mutation
    W105M
  • GenBank ID
    NM_001260.1
  • Entrez Gene
    CDK8 (a.k.a. IDDHBA, K35)
  • Promoter EF1α
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pBabe.puro.CDK8.flag (Plasmid #19758) Mutagenized W105M
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Flag-CDK8-W105M-IRES-mCherry was a gift from Matthew Shair (Addgene plasmid # 68857 ; http://n2t.net/addgene:68857 ; RRID:Addgene_68857)

  • For your References section:

    Mediator kinase inhibition further activates super-enhancer-associated genes in AML. Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, Taatjes DJ, Shair MD. Nature. 2015 Sep 28. doi: 10.1038/nature14904. 10.1038/nature14904 PubMed 26416749
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