pCS6-YFP-SMASh
(Plasmid #68853)

• Purpose: Expresses SMASh tag fused to YFP at C terminus in mammalian cells
• Depositing Lab: Michael Lin
• Publication: Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869.

Backbone

• Vector backbone: pCS6
• Backbone size w/o insert (bp): 6622
• Total vector size (bp): 8260
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YFP-SMASh
• Species: HCV, Aequorea victoria
• Insert Size (bp): 1638
• Promoter: CMV
• Tag / Fusion Protein:
  • SMASh tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTTAGGTGACACTATAG
• 3′ sequencing primer: CAGGCTGGGTCCTGTCACTGGCTAC

Resource Information

• Supplemental Documents:
  • pCS6-YFP-SMASh GenBank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry