pQ398X-EGFP
(Plasmid #68849)

• Purpose: mutated OPTN was inserted into pEGFP-N1 backbone
• Depositing Lab: Beatrice Yue
• Publication: Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.1186/2193-1801-3-99. eCollection 2014.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: optineurin
• Alt name: OPTN
• Species: H. sapiens (human)
• Mutation: Q398X, results in truncation of the protein into a 1-398 fragment
• Entrez Gene: OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Wild type construct is pOPTN-EGFP (Plasmid #27052)