pOPTN425-577-EGFP
(Plasmid
#68843)
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Purposedeleted OPTN was inserted into pEGFP-N1 backbone
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameoptineurin
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Alt nameOPTN
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SpeciesH. sapiens (human)
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Mutationaa 425-577 only
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Entrez GeneOPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Wild type construct is pOPTN-EGFP (Plasmid #27052)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPTN425-577-EGFP was a gift from Beatrice Yue (Addgene plasmid # 68843 ; http://n2t.net/addgene:68843 ; RRID:Addgene_68843) -
For your References section:
Effects of mutations and deletions in the human optineurin gene. Turturro S, Shen X, Shyam R, Yue BY, Ying H. Springerplus. 2014 Feb 19;3:99. doi: 10.1186/2193-1801-3-99. eCollection 2014. 845 [pii] PubMed 24683533