pAnc80L65
(Plasmid #68837)

• Purpose: Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
• Depositing Lab: Luk Vandenberghe
• Publication: Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 10.1016/j.celrep.2015.07.019.

Backbone

• Vector backbone: synthetic
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 7446
• Vector type: Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Growth instructions: Stbl4 is also appropriate for growth.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ancestral AAV Capsid Anc80L65
• Alt name: ANC80L65
• Species: Synthetic
• Insert Size (bp): 2208
• Promoter: Rep2

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An improved version of this plasmid from the Vandenberghe Lab is available. Please see pAnc80L65AAP (Addgene plasmid# 92307).

This plasmid, pAnc80L65 (#68837), requires cotransfection with a plasmid expressing AAP. For efficient viral vector production without cotransfecting AAP, use pAnc80L65AAP (Addgene plasmid# 92307)