DIG70 (T7->Rci; 11bc)
(Plasmid #68835)

• Purpose: Expresses Rci gene (driven by the inducible T7 promoter) and 11 fragment barcode cassette in bacterial cells
• Depositing Lab: Anthony Zador
• Publication: Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi: 10.1093/nar/gku604. Epub 2014 Jul 10.

Backbone

• Vector backbone: pet22b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5433
• Total vector size (bp): 8060

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Barcode cassette 11 fragments
• Insert Size (bp): 1472

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: na

Gene/Insert 2

• Gene/Insert name: Rci recombinase
• Alt name: Rci
• Insert Size (bp): 1155
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • dig70 annotated GenBank file
• A portion of this plasmid was derived from a plasmid made by: ORF of Rci was obtained by PCR of pEK204 plasmid

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry