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Addgene

hRAD18-EGFP
(Plasmid #68824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human RAD18 tagged with EGFP
  • Alt name
    hRAD18-EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2210
  • Entrez Gene
    RAD18 (a.k.a. RNF73)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer 5' primer for pEGFP-N3
  • 3′ sequencing primer 3' primer for pEGFP-N3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hRAD18-EGFP was a gift from Satoshi Tateishi (Addgene plasmid # 68824 ; http://n2t.net/addgene:68824 ; RRID:Addgene_68824)

  • For your References section:

    Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Tsuji Y, Watanabe K, Araki K, Shinohara M, Yamagata Y, Tsurimoto T, Hanaoka F, Yamamura K, Yamaizumi M, Tateishi S. Genes Cells. 2008 Apr;13(4):343-54. doi: 10.1111/j.1365-2443.2008.01176.x. 10.1111/j.1365-2443.2008.01176.x PubMed 18363965
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