EGFPC2-CB2 SH3+
(Plasmid #68821)

• Purpose: EGFP tagged Collybistin expression in mammalian cells
• Depositing Lab: Shiva Tyagarajan
• Publication: de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073. doi: 10.1371/journal.pgen.1007073. eCollection 2017 Oct.

Backbone

• Vector backbone: pEGFP C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Collybistin
• Species: R. norvegicus (rat)
• Insert Size (bp): 1236
• Mutation: none
• Entrez Gene: Arhgef9
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CB2SH3+ contains the SH3 domain splice cassette, CB2SH3- lacks the Sh3 domain splice cassette. That is the only difference between these two splice isoforms.