pmCherryC2-Gephyrin P1
(Plasmid #68820)

• Purpose: mCherry tagged Geph for mammalian expression
• Depositing Lab: Shiva Tyagarajan
• Publication: Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):379-84. doi: 10.1073/pnas.1011824108. Epub 2010 Dec 20.

Backbone

• Vector backbone: pEGFP C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gephyrin
• Alt name: GPHN
• Species: R. norvegicus (rat)
• Insert Size (bp): 2200
• Mutation: silent mutations on internal EcoRI site. Gephyrin D566N (see depositor comment below)
• GenBank ID: NM022865.3
• Entrez Gene: Gphn (a.k.a. Geph)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP was removed using NheI and XhoI. mCherry was amplified and inserted using NheI and XhoI sites. Gephyrin D566N mutation is a polymorphism and is not a concern for plasmid function.