pTriEx4-Cdc42-2G
(Plasmid #68814)

• Purpose: FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
• Depositing Lab: Olivier Pertz
• Publication: Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.

Backbone

• Vector backbone: pTriEx4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 7608
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cdc42 activation reporter
• Species: Synthetic
• Insert Size (bp): 2670
• Promoter: CMV
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ctggttattgtgctgtctc
• 3′ sequencing primer: tcagtggtatttgtga

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry