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Addgene

pLenti-Cdc42-2G
(Plasmid #68813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL- SV40(puro)_CMV(mcs)
  • Backbone size w/o insert (bp) 7714
  • Total vector size (bp) 10424
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cdc42 activation reporter
  • Species
    Synthetic
  • Insert Size (bp)
    2669
  • Promoter CMV
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGACTCTAGAGGATCCggagatatA
  • 3′ sequencing primer CTAGACTGCAGGATCCTGGTGATGGTGATGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Cdc42-2G was a gift from Olivier Pertz (Addgene plasmid # 68813 ; http://n2t.net/addgene:68813 ; RRID:Addgene_68813)

  • For your References section:

    Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation during prototypical edge protrusion and retraction dynamics. Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O. Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901. 10.1038/srep21901 PubMed 26912264
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