pLenti-Cdc42-2G
(Plasmid #68813)

• Purpose: FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)
• Depositing Lab: Olivier Pertz
• Publication: Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.

Backbone

• Vector backbone: pRRL- SV40(puro)_CMV(mcs)
• Backbone size w/o insert (bp): 7714
• Total vector size (bp): 10424
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cdc42 activation reporter
• Species: Synthetic
• Insert Size (bp): 2669
• Promoter: CMV
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CGACTCTAGAGGATCCggagatatA
• 3′ sequencing primer: CTAGACTGCAGGATCCTGGTGATGGTGATGG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry