pCW57.1-FOXM1b
(Plasmid #68811)

• Purpose: Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells
• Depositing Lab: Adam Karpf
• Publication: Barger et al Oncotarget. 2015 Jul 16.

Backbone

• Vector backbone: pCW57.1
• Backbone manufacturer: Broad Insitute
• Backbone size w/o insert (bp): 7667
• Total vector size (bp): 10061
• Vector type: Mammalian Expression, Lentiviral ; Doxycycline inducible
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FOXM1b
• Alt name: Forkhead Box M1b
• Alt name: FOXM1
• Alt name: Forkhead Box M1
• Species: H. sapiens (human)
• Insert Size (bp): 2407
• GenBank ID: NM_202003.2
• Entrez Gene: FOXM1 (a.k.a. FKHL16, FOXM1A, FOXM1B, FOXM1C, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TRIDENT)
• Promoter: TRE promoter, Tet ON
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX (5'-AGCTCGTTTAGTGAACCGTCAGATC-3')
• 3′ sequencing primer: O.PGK1b-R (5'-GAACGGACGTGAAGAATGTG-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The human FOXM1b open reading frame was subcloned from Origene FOXM1b cDNA construct (Cat SC128214 ) into Origene (Cat PS100013) pCMV6-AN-HA then finally subcloned into pCW57.1.
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The plasmid is derived from a FOXM1b cDNA clone from Origene which contains a SNP which causes a serine to proline amino acid residue substitution. This residue change is a natural variant and does not affect plasmid function.