pGL4.26-SS-352
(Plasmid #68791)

• Purpose: ZF9 Op x6 -- GFP-IRES-NTR
• Depositing Lab: James Collins
• Publication: Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.

Backbone

• Vector backbone: pGL4.26 luc2/minp/hygro
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4917
• Total vector size (bp): 6127
• Modifications to backbone: Luc replaced with GFP-IRES-NTR
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ZF9 Op 6x
• Species: Synthetic
• Insert Size (bp): 81

Gene/Insert 2

• Gene/Insert name: EGFP
• Species: Synthetic
• Insert Size (bp): 720
• Tag / Fusion Protein:
  • IRES (C terminal on insert)

Gene/Insert 3

• Gene/Insert name: Nitroreductase
• Alt name: NTR
• Species: E. Coli (human codon optimized)
• Insert Size (bp): 654
• Tag / Fusion Protein:
  • IRES (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry