pGL4.26-SS-358
(Plasmid
#68790)
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PurposeZF9 Op x6 -- NTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.26 luc2/minp/hygro
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4917
- Total vector size (bp) 4935
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Modifications to backboneLuc replaced by NTR
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameZF9 Op x6
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SpeciesSynthetic
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Insert Size (bp)81
Gene/Insert 2
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Gene/Insert nameNitroreductase
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Alt nameNTR
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SpeciesE. Coli (mammalian codon optimized)
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Insert Size (bp)657
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.26-SS-358 was a gift from James Collins (Addgene plasmid # 68790 ; http://n2t.net/addgene:68790 ; RRID:Addgene_68790) -
For your References section:
DNA sense-and-respond protein modules for mammalian cells. Slomovic S, Collins JJ. Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585. 10.1038/nmeth.3585 PubMed 26389572