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Addgene

pGL4.26-SS-358
(Plasmid #68790)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68790 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL4.26 luc2/minp/hygro
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4917
  • Total vector size (bp) 4935
  • Modifications to backbone
    Luc replaced by NTR
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ZF9 Op x6
  • Species
    Synthetic
  • Insert Size (bp)
    81

Gene/Insert 2

  • Gene/Insert name
    Nitroreductase
  • Alt name
    NTR
  • Species
    E. Coli (mammalian codon optimized)
  • Insert Size (bp)
    657

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.26-SS-358 was a gift from James Collins (Addgene plasmid # 68790 ; http://n2t.net/addgene:68790 ; RRID:Addgene_68790)
  • For your References section:

    DNA sense-and-respond protein modules for mammalian cells. Slomovic S, Collins JJ. Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585. 10.1038/nmeth.3585 PubMed 26389572