pVITRO1-SS-288
(Plasmid
#68788)
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PurposeN Trans V2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVITRO1-NEO-MCS
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Backbone manufacturerinvivogen
- Backbone size w/o insert (bp) 6295
- Total vector size (bp) 7793
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN-Terminal Sensor 1 V2
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SpeciesSynthetic
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Insert Size (bp)1494
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Tags
/ Fusion Proteins
- flag (N terminal on insert)
- NLS (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVITRO1-SS-288 was a gift from James Collins (Addgene plasmid # 68788 ; http://n2t.net/addgene:68788 ; RRID:Addgene_68788) -
For your References section:
DNA sense-and-respond protein modules for mammalian cells. Slomovic S, Collins JJ. Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585. 10.1038/nmeth.3585 PubMed 26389572