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Addgene

pBW121-SS-321
(Plasmid #68787)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68787 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBW121
  • Backbone size w/o insert (bp) 4838
  • Total vector size (bp) 5182
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8x 0gap Target 2 with SV40 Ori
  • Insert Size (bp)
    344

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains single-base deletion mutation in Target #3 and Target #7 discovered via sequencing

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBW121-SS-321 was a gift from James Collins (Addgene plasmid # 68787 ; http://n2t.net/addgene:68787 ; RRID:Addgene_68787)
  • For your References section:

    DNA sense-and-respond protein modules for mammalian cells. Slomovic S, Collins JJ. Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585. 10.1038/nmeth.3585 PubMed 26389572