pBW121-SS-273
(Plasmid
#68763)
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PurposeZF9-TF-V2 with N-FLAG and C-HIS
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBW121
- Backbone size w/o insert (bp) 4504
- Total vector size (bp) 5065
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZF9-TF-V2
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SpeciesSynthetic
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Insert Size (bp)561
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Tags
/ Fusion Proteins
- flag (N terminal on insert)
- NLS (N terminal on insert)
- HIS (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBW121-SS-273 was a gift from James Collins (Addgene plasmid # 68763 ; http://n2t.net/addgene:68763 ; RRID:Addgene_68763) -
For your References section:
DNA sense-and-respond protein modules for mammalian cells. Slomovic S, Collins JJ. Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585. 10.1038/nmeth.3585 PubMed 26389572
