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Addgene

pVITRO1-SS-127
(Plasmid #68733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVITRO1-NEO-MCS
  • Backbone manufacturer
    invivogen
  • Backbone size w/o insert (bp) 6295
  • Total vector size (bp) 7477
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ZF5-VP64
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • flag (N terminal on insert)
    • NLS (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    708

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVITRO1-SS-127 was a gift from James Collins (Addgene plasmid # 68733 ; http://n2t.net/addgene:68733 ; RRID:Addgene_68733)

  • For your References section:

    DNA sense-and-respond protein modules for mammalian cells. Slomovic S, Collins JJ. Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585. 10.1038/nmeth.3585 PubMed 26389572
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