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pMpGWB421
(Plasmid #68686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMpGWB400
  • Backbone manufacturer
    Kohchi Lab., Kyoto University
  • Backbone size (bp) 9287
  • Modifications to backbone
    MpEF1alpha promoter and coding sequence for a fusion polypeptide between the modified EAR motif plant-specific repression domain showing strong repression activity (SRDX) and glucocorticoid receptor (GR) are cloned upstream and downstream of the Gateway cassette, respectively.
  • Vector type
    Plant Expression
  • Promoter MpEF1alpha promoter
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • Modified EAR motif plant-specific repression domain showing strong repression activity (SRDX) and glucocorticoid receptor (GR) (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Gateway cassette was provided by Tsuyoshi Nakagawa at Shimane University, Japan.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGWB421 was a gift from Takayuki Kohchi (Addgene plasmid # 68686 ; http://n2t.net/addgene:68686 ; RRID:Addgene_68686)

  • For your References section:

    Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T. PLoS One. 2015 Sep 25;10(9):e0138876. doi: 10.1371/journal.pone.0138876. eCollection 2015. PONE-D-15-25288 [pii] PubMed 26406247
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