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Addgene

pMpGWB300
(Plasmid #68628)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68628 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGWB400
  • Backbone manufacturer
    Tsuyoshi Nakagawa, Shimane University
  • Backbone size (bp) 9135
  • Modifications to backbone
    The proNOS:nptII:terNOS marker cassette was replaced with pro35Sx2:mALS:ter35S.
  • Vector type
    Plant Expression
  • Selectable markers
    Chlorsulfuron

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGWB300 was a gift from Takayuki Kohchi (Addgene plasmid # 68628 ; http://n2t.net/addgene:68628 ; RRID:Addgene_68628)
  • For your References section:

    Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T. PLoS One. 2015 Sep 25;10(9):e0138876. doi: 10.1371/journal.pone.0138876. eCollection 2015. PONE-D-15-25288 [pii] PubMed 26406247