pMpGWB227
(Plasmid
#68618)
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Purpose(Empty Backbone) Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68618 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMpGWB200
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Backbone manufacturerKohchi Lab., Kyoto University
- Backbone size (bp) 9021
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Modifications to backboneMpEF1alpha promoter and coding sequence for TagRFP are cloned upstream and downstream of the Gateway cassette, respectively.
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Vector typePlant Expression
- Promoter MpEF1alpha promoter
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Selectable markersGentamicin
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Tag
/ Fusion Protein
- TagRFP (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe Gateway cassette was provided by Tsuyoshi Nakagawa at Shimane University, Japan.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMpGWB227 was a gift from Takayuki Kohchi (Addgene plasmid # 68618 ; http://n2t.net/addgene:68618 ; RRID:Addgene_68618) -
For your References section:
Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T. PLoS One. 2015 Sep 25;10(9):e0138876. doi: 10.1371/journal.pone.0138876. eCollection 2015. PONE-D-15-25288 [pii] PubMed 26406247
Map uploaded by the depositor.
