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Myc-Idax DBM
(Plasmid #68554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6174
  • Total vector size (bp) 6810
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IDAX-DBM
  • Alt name
    Cxxc4-DBM
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    636
  • Mutation
    T162A, H164A and Q165A mutations
  • GenBank ID
    NM_001004367.3 NP_001004367.1
  • Entrez Gene
    Cxxc4 (a.k.a. 9330210J02Rik, C030003J12Rik, Idax)
  • Promoter EF1a
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-Idax DBM was a gift from Anjana Rao (Addgene plasmid # 68554 ; http://n2t.net/addgene:68554 ; RRID:Addgene_68554)

  • For your References section:

    Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX. Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A. Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 2013 Apr 7. 10.1038/nature12052 PubMed 23563267
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