CLAG-bio
(Plasmid #68531)

• Purpose: Expresses full-length P. vivax CLAG (PVX_121885) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
• Depositing Labs: Julian Rayner, Gavin Wright
• Publication: Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 10.1371/journal.pntd.0004264. eCollection 2015 Dec.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6654
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CLAG
• Alt name: PVX_121885
• Mutation: All NXT/S sites mutated to NXA, where X is any amino acid except Proline
• Promoter: CMV
• Tag / Fusion Protein:
  • rat CD4 d3+4, enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: pMT2-F
• 3′ sequencing primer: rb_glob_PA_term-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry