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Addgene

AAV_NLS-dSaCas9-NLS-VPR Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV_NLS-dSaCas9-NLS-VPR with the following enzymes: XbaI and EcoRI-HF.

Expected:
XbaI - 8.6 kb
XbaI/EcoRI-HF - 7.2 + 1.4 kb.

These results show the expected band pattern.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV_NLS-dSaCas9-NLS-VPR  with the following enzymes: EcoRI-HF/HindIII-HF

We observed the expected bands for this digest