AAV_NLS-dSaCas9-NLS-VPR Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV_NLS-dSaCas9-NLS-VPR with the following enzymes: XbaI and EcoRI-HF.
Expected:
XbaI - 8.6 kb
XbaI/EcoRI-HF - 7.2 + 1.4 kb.
These results show the expected band pattern.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid AAV_NLS-dSaCas9-NLS-VPR with the following enzymes: EcoRI-HF/HindIII-HF
We observed the expected bands for this digest