AAV_NLS-dSaCas9-NLS-VPR
(Plasmid #68495)

• Purpose: AAV vector containing nuclease null SaCas9 fused to VPR
• Depositing Lab: George Church
• Publication: Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.

Backbone

• Vector backbone: pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA Plasmid #61592
• Total vector size (bp): 8613
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dSaCas9
• Alt name: dSauCas9
• Species: Synthetic; S. aureus
• Insert Size (bp): 4857
• Promoter: CMV
• Tag / Fusion Protein:
  • VPR (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTACTACGAAGTGAATAGCAAGTGC
• 3′ sequencing primer: ACTCAGACAATGCGATGCAA

Resource Information

• Supplemental Documents:
  • nuclease null SaCas9-VPR
• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry