MSCV Puro-EFS:GFP
(Plasmid #68484)

• Purpose: Retrovirus expressing GFP driven by EFS promoter
• Depositing Lab: Tyler Jacks
• Publication: Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.

Backbone

• Vector backbone: MSCV 2-5
• Backbone size w/o insert (bp): 5160
• Total vector size (bp): 6800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Puromycin Resistance
• Alt name: PuroR
• Insert Size (bp): 603
• Promoter: LTR

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTAACTCGAACGCTAGCTGTGCGATCGTTT
• 3′ sequencing primer: AGGCCTCGGGATTCCTAGGAACAGCGGTTT

Gene/Insert 2

• Gene/Insert name: GFP
• Insert Size (bp): 723
• Promoter: EFS

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACCGCTGTTCCTAGGAATCCCGAGGCCT
• 3′ sequencing primer: AGAGTAATTCAACCCCAAACAACAACGTTT

Resource Information

• Supplemental Documents:
  • MacVector Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry