Skip to main content
Addgene

pGK:HygroR-CMV:mKO2-C_UTR
(Plasmid #68481)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68481 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    LV 1-5
  • Backbone size w/o insert (bp) 5818
  • Total vector size (bp) 8816
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Hygromycin Resistance
  • Alt name
    HygroR
  • Insert Size (bp)
    1008
  • Promoter pGK

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATCAGTGTGAGGGAGTGTAAAGCTGGTTT
  • 3′ sequencing primer AGGCCTCGGGATTCCTAGGAACAGCGGTTT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mKO2-C_UTR
  • Insert Size (bp)
    722
  • Mutation
    shRNA sensor cassette "B" added to 3'UTR
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACCGCTGTTCCTAGGAATCCCGAGGCCT
  • 3′ sequencing primer AGAGTAATTCAACCCCAAACAACAACGTTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGK:HygroR-CMV:mKO2-C_UTR was a gift from Tyler Jacks (Addgene plasmid # 68481 ; http://n2t.net/addgene:68481 ; RRID:Addgene_68481)

  • For your References section:

    A Modular Assembly Platform for Rapid Generation of DNA Constructs. Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T. Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. 10.1038/srep16836 PubMed 26887506
Related items:
Commonly requested with: