pGK:HygroR-CMV:mKate2-B_UTR
(Plasmid #68480)

• Purpose: Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance
• Depositing Lab: Tyler Jacks
• Publication: Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.

Backbone

• Vector backbone: LV 1-5
• Backbone size w/o insert (bp): 5818
• Total vector size (bp): 8852
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Hygromycin Resistance
• Alt name: HygroR
• Insert Size (bp): 1008
• Promoter: pGK

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATCAGTGTGAGGGAGTGTAAAGCTGGTTT
• 3′ sequencing primer: AGGCCTCGGGATTCCTAGGAACAGCGGTTT

Gene/Insert 2

• Gene/Insert name: mKate2-B_UTR
• Insert Size (bp): 749
• Mutation: shRNA sensor cassette "B" added to 3'UTR
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACCGCTGTTCCTAGGAATCCCGAGGCCT
• 3′ sequencing primer: AGAGTAATTCAACCCCAAACAACAACGTTT

Resource Information

• Supplemental Documents:
  • MacVector Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry