pTRE:CloverCP-EFS:ZeoR
(Plasmid #68478)

• Purpose: Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP
• Depositing Lab: Tyler Jacks
• Publication: Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.

Backbone

• Vector backbone: LV 1-5
• Backbone size w/o insert (bp): 5818
• Total vector size (bp): 7840
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CloverCP
• Insert Size (bp): 936
• Promoter: pTRE
• Tag / Fusion Protein:
  • "CP" degradation domain (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATCAGTGTGAGGGAGTGTAAAGCTGGTTT
• 3′ sequencing primer: AGGCCTCGGGATTCCTAGGAACAGCGGTTT

Gene/Insert 2

• Gene/Insert name: Zeocin Resistance
• Alt name: ZeoR
• Insert Size (bp): 375
• Promoter: EFS

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACCGCTGTTCCTAGGAATCCCGAGGCCT
• 3′ sequencing primer: AGAGTAATTCAACCCCAAACAACAACGTTT

Resource Information

• Supplemental Documents:
  • MacVector Map
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry