pGK:TRL-EFS:iRFP670
(Plasmid #68475)

• Purpose: Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670
• Depositing Lab: Tyler Jacks
• Publication: Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.

Backbone

• Vector backbone: LV 1-5
• Backbone size w/o insert (bp): 5818
• Total vector size (bp): 11091
• Vector type: Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: tTR-KRAB-2A-rtTA3-2A-Luciferase
• Species: H. sapiens (human); Escherichia coli Tn10
• Insert Size (bp): 3549
• Promoter: pGK

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTAACTCGAACGCTAGCTGTGCGATCGTTT
• 3′ sequencing primer: AGAGTAATTCAACCCCAAACAACAACGTTT

Gene/Insert 2

• Gene/Insert name: iRFP670
• Insert Size (bp): 939
• Promoter: EFS

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACGTTGTTGTTTGGGGTTGAATTACTCTTCTAGACCGCGG
• 3′ sequencing primer: TGTAATCCAGAGGTTGATTATCGGG

Resource Information

• Supplemental Documents:
  • MacVector Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry