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Addgene

pTRE:shRNA A-pGK:rtTA-Cre
(Plasmid #68471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LV 1-5
  • Backbone size w/o insert (bp) 5818
  • Total vector size (bp) 10158
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GFP-shRNA A-miR30
  • Insert Size (bp)
    1534
  • Promoter pTRE

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATCAGTGTGAGGGAGTGTAAAGCTGGTTT
  • 3′ sequencing primer AGGCCTCGGGATTCCTAGGAACAGCGGTTT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    rtTA-2A-Cre
  • Insert Size (bp)
    1851
  • Promoter pGK

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer AAACCGCTGTTCCTAGGAATCCCGAGGCCT
  • 3′ sequencing primer AGAGTAATTCAACCCCAAACAACAACGTTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE:shRNA A-pGK:rtTA-Cre was a gift from Tyler Jacks (Addgene plasmid # 68471 ; http://n2t.net/addgene:68471 ; RRID:Addgene_68471)

  • For your References section:

    A Modular Assembly Platform for Rapid Generation of DNA Constructs. Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T. Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. 10.1038/srep16836 PubMed 26887506
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