FFiBlast MSCV Puro
(Plasmid
#68464)
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PurposeRetroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV pGK Puro
- Backbone size w/o insert (bp) 6295
- Total vector size (bp) 6983
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Vector typeMammalian Expression, Retroviral, Cre/Lox
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Selectable markersPuromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBlasticidin Resistance
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Alt nameBlastR
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Insert Size (bp)402
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MutationInverted
- Promoter LTR
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGCCGGAATTAGATCTCTCGAGGTCGACGGTATCG
- 3′ sequencing primer GTAGAATTCGTTAACCTCGATCTAGACTCGAGCGGCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FFiBlast MSCV Puro was a gift from Tyler Jacks (Addgene plasmid # 68464 ; http://n2t.net/addgene:68464 ; RRID:Addgene_68464) -
For your References section:
A Modular Assembly Platform for Rapid Generation of DNA Constructs. Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T. Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. 10.1038/srep16836 PubMed 26887506