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Addgene

AAVS1-Neo-CAG-Flpe-ERT2 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid #68460 with the enzymes Age I-HF and Kpn I-HF.

The expected pattern of bands was observed:

AgeI-HF single digest: linearized plasmid ~13kb
KpnI-HF single digest: linearized plasmid ~13kb
AgeI-HF / KpnI-HF double digest: 3kb and 10kb bands