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Addgene

TOPO pGK
(Plasmid #68452)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TOPO 1-4
  • Backbone size w/o insert (bp) 3519
  • Vector type
    Bacterial Expression ; GMAP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Phosphoglycerate Kinase 1 Promoter
  • Alt name
    pGK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    505
  • Entrez Gene
    PGK1 (a.k.a. HEL-S-68p, MIG10, PGKA)
  • Promoter Plac

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATCAGTGTGAGGGAGTGTAAAGCTGGTTT
  • 3′ sequencing primer CCGCTTAAGCTGTAGCGCTAATGTCGGGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TOPO pGK was a gift from Tyler Jacks (Addgene plasmid # 68452 ; http://n2t.net/addgene:68452 ; RRID:Addgene_68452)

  • For your References section:

    A Modular Assembly Platform for Rapid Generation of DNA Constructs. Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T. Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. 10.1038/srep16836 PubMed 26887506
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