pUBC_HA_2xPP7-VP64
(Plasmid #68418)

• Purpose: Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
• Depositing Lab: John Rinn
• Publication: Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.

Backbone

• Vector backbone: pNEB193
• Backbone manufacturer: NEB
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PP7(deltaFG)
• Mutation: PP7: [delta]67_74 (non-aggregating)
• Promoter: human UbC
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • VP64 (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: hUBCpro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pUBC_HA_2xPP7-VP64 genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains an N-terminal 3xNLS cassette. Contains two tandem copies of the PP7 protein, connected by a flexible linker