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Addgene

Cav3P104L-YFP
(Plasmid #68404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4856
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cav3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    456
  • Mutation
    Proline at position 104 changed to a Leucine
  • Entrez Gene
    Cav3 (a.k.a. Cav-3, M-cav)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer EGFPN forward
  • 3′ sequencing primer eGFPN reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This has been made by cloning Cav3P104L from HA tagged Cav3P104L construct as described in Carozzi et al 2002

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cav3P104L-YFP was a gift from Rob Parton (Addgene plasmid # 68404 ; http://n2t.net/addgene:68404 ; RRID:Addgene_68404)

  • For your References section:

    Cholesterol and fatty acids regulate dynamic caveolin trafficking through the Golgi complex and between the cell surface and lipid bodies. Pol A, Martin S, Fernandez MA, Ingelmo-Torres M, Ferguson C, Enrich C, Parton RG. Mol Biol Cell. 2005 Apr;16(4):2091-105. Epub 2005 Feb 2. 10.1091/mbc.e04-08-0737 PubMed 15689493
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