Skip to main content
Addgene

pEGFP-PTRF
(Plasmid #68401)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5880
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTRF
  • Alt name
    Cavin1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1180
  • Entrez Gene
    Cavin1 (a.k.a. 2310075E07Rik, Cav-p60, Cavin, Ptrf)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer EGFPN forward
  • 3′ sequencing primer eGFPN reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-PTRF was a gift from Rob Parton (Addgene plasmid # 68401 ; http://n2t.net/addgene:68401 ; RRID:Addgene_68401)

  • For your References section:

    PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function. Hill MM, Bastiani M, Luetterforst R, Kirkham M, Kirkham A, Nixon SJ, Walser P, Abankwa D, Oorschot VM, Martin S, Hancock JF, Parton RG. Cell. 2008 Jan 11;132(1):113-24. doi: 10.1016/j.cell.2007.11.042. 10.1016/j.cell.2007.11.042 PubMed 18191225
Related items:
Commonly requested with: