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Addgene

GFP SRBC
(Plasmid #68399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5957
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRBC
  • Alt name
    Cavin3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    784
  • Entrez Gene
    Cavin3 (a.k.a. 3110015B12Rik, 6330514M23Rik, Prkcdbp, SRBC)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer EGFPC forward
  • 3′ sequencing primer eGFPC reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP SRBC was a gift from Rob Parton (Addgene plasmid # 68399 ; http://n2t.net/addgene:68399 ; RRID:Addgene_68399)

  • For your References section:

    MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. Bastiani M, Liu L, Hill MM, Jedrychowski MP, Nixon SJ, Lo HP, Abankwa D, Luetterforst R, Fernandez-Rojo M, Breen MR, Gygi SP, Vinten J, Walser PJ, North KN, Hancock JF, Pilch PF, Parton RG. J Cell Biol. 2009 Jun 29;185(7):1259-73. doi: 10.1083/jcb.200903053. Epub 2009 Jun 22. 10.1083/jcb.200903053 PubMed 19546242
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