GFP SDPR
(Plasmid
#68398)
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PurposeExpression of fluorescently tagged SDPR in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5957
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSDPR
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Alt nameCavin2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1258
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Entrez GeneSdpr
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer EGFPC forward
- 3′ sequencing primer EGFPC reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP SDPR was a gift from Rob Parton (Addgene plasmid # 68398 ; http://n2t.net/addgene:68398 ; RRID:Addgene_68398) -
For your References section:
MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. Bastiani M, Liu L, Hill MM, Jedrychowski MP, Nixon SJ, Lo HP, Abankwa D, Luetterforst R, Fernandez-Rojo M, Breen MR, Gygi SP, Vinten J, Walser PJ, North KN, Hancock JF, Pilch PF, Parton RG. J Cell Biol. 2009 Jun 29;185(7):1259-73. doi: 10.1083/jcb.200903053. Epub 2009 Jun 22. 10.1083/jcb.200903053 PubMed 19546242