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PurposeFluorescently tagged Caveolin-3 for expression in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5153
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCav3
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Alt nameCaveolin3
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SpeciesM. musculus (mouse)
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Insert Size (bp)453
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GenBank IDNM_007617
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Entrez GeneCav3 (a.k.a. Cav-3, M-cav)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer eGFPC forward
- 3′ sequencing primer eGFPC reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Caveolin-3 was a gift from Rob Parton (Addgene plasmid # 68396 ; http://n2t.net/addgene:68396 ; RRID:Addgene_68396) -
For your References section:
Molecular characterization of caveolin association with the Golgi complex: identification of a cis-Golgi targeting domain in the caveolin molecule. Luetterforst R, Stang E, Zorzi N, Carozzi A, Way M, Parton RG. J Cell Biol. 1999 Jun 28;145(7):1443-59. PubMed 10385524